1: Minion wall with Mage healer and some Spiders wherever.
2:Place main buff beam vertical
First Wave build Auras, buff beams, and as many towers as possible
For towers try to aim 2 towards top left crystal one towards middle and rest towards little stack with ogre to help with boss and mobs on right side.
Tiips: Queen spawns bunch of wasps that can damage crystal so dont fight her too close to it or they will hit it and make you lose.
Tips: Stacking Summoner minions on buff beam and try to use it sparingly basically just there if you ever need a second to heal or disengage for any reason.
Tips: Keep TB monk going almost all times during waves and boss fight, use upgrader and Jester to get everything 3 star by wave 4
I did solo with most of the time having TB monk, Jester, Upgrader in. For boss fight I did Tb Monk, Jester Dps, Builder app, App/ Huntress Guardian Booster (~90-100)